The sensitivity of Quantitative Polymerase Chain Reaction (qPCR) assays is one of the most important reasons for their success and abundant use in scientific laboratories. However, many factors including pipetting technique can influence qPCR assay results. PCR Master mix is routinely used during qPCR set-up but can be challenging to pipette accurately. Sartorius has undertaken a study to determine the best technique for pipetting Master mix in qPCR assays. Forward and reverse pipetting techniques, the type of pipette tips, pre-wetting of the pipette tip, and the use of electronic pipettes were evaluated. 

The full study is available as a free download here.

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