• Global food and agriculture company partners with Bio-Rad Laboratories, Inc. to evaluate methods for detecting Listeria spp. in environmental sponge samples.
• Initial testing showed positive results after 4-5 hours with pre-warmed media.
• Download the application note to learn more.

A global food and agriculture company partnered with Bio-Rad Laboratories, Inc. to evaluate methods to detect Listeria spp. in environmental sponge samples with a target 6-12 hour sample enrichment period to obtain quantitative PCR (qPCR) results in one day.

Current methods typically require 24 hours of incubation. In previous studies, we identified the minimum incubation period required to detect multiple strains of Listeria at an inoculum of 30-50 colony forming units (CFU) with the iQ-Check™ Listeria spp. PCR Detection Kit method with and without preheating the enrichment media.

Initial testing showed that with pre-warmed media, positive results for Listeria spp. can be obtained following a 4-5 hour incubation period with the iQ-Check Standard II Protocol. Environmental sponges were obtained from a manufacturing production plant containing normal flora for subsequent inoculation with Listeria in the laboratory to challenge the method further and simulate real-world conditions. A new highly nutritive enrichment medium, Listeria Special Broth II (LSB II), was tested and facilitated the reduced enrichment time.

Click here to download the full application note or visit the product page.