This kit is used for the qualitative detection of novel coronavirus IgG antibodies in human serum or plasma in vitro.

Principle: This kit is based on the capture ELISA method to detect the SARS-COV-2 IgM antibody in human serum or plasma. The anti-µ chain monoclonal antibody is pre-coated on the microplate wells. Add the test sample and incubate it. The IgM antibody in the sample is bound to anti-µ chain monoclonal antibody. Wash the plate to remove SARS-COV-2 IgM that does not bind to the microplate wells. Add the enzyme-labelled reagent for a second incubation.

When a SARS-CoV-2 IgM antibody is present in the sample, an "anti-µ chain monoclonal antibody-human anti nCoV IgM antibody-enzyme conjugated antigen" complex will be formed. After rewashing the plate, color reagent is added, and the HRP linked to the complex will catalyze the color. The reagent reacts to produce a blue product, which turns yellow after the reaction is terminated; if no SARS-COV-2 IgM antibody is present in the sample, it does not develop a color. Measure the OD value on a microplate reader or enzyme immunoassay system, and determine the presence or absence of a SARS-COV-2 IgM antibody based on the OD value.

• High-sensitivity ELISA enables detection of low amounts of SARS-CoV-2 IgM
• Rapid qualitative results in under 2 hours
• High-throughput immunoassay measures up to 86 samples per kit