Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is an essential tool utilised across the field of microbiology, whether it be the detection of pathogens, gene expression analysis or the detection of antimicrobial resistance genes. The same issues hamper the technique and frustrate microbiologists: lack of sensitivity and specificity, the presence of carryover contamination, slow cycling times and mastermixes uncapable of multiplexing diminish the reliability of RT-qPCR. Utilising our 25+ years of experience, Microzone have developed MegaMix Platinum Probe RT-qPCR that offers unrivaled, confidence inspiring performance for multiplexed probe-based RNA applications, perfect for microbiologists looking to detect or quantify RNA from any source.

Microzone’s MegaMix Platinum Probe RT-qPCR has been optimised to allow the sensitive and specific detection of multiple targets in one reaction. Its suitability for probe detection (such as Taqman probes) means that existing assays can be easily converted and benefit from its enhanced performance. Its rapid one-step format means that not only does the user not need to generate cDNA separately, but the entire RT-qPCR can take less than 45 minutes!

Key Features:

• Highly Specific - hot-start Taq polymerase in specificity enhancing buffer guarantees reliable amplification of a range a of RNA targets.
• Highly Sensitive - optimised for single copy detection.
• Multiplexing - excellent for multiplexed reactions consisting of 4 or 5 targets without compromising performance.
• Eliminates carry over contamination - incorporation thermolabile UNG and dUTP prevent amplicon contamination from previous runs.
• Versatile template compatibility - compatible with GC/AT rich templates.
• Rapid – fast cycling times less than 45 minutes for RT-qPCR.

  Benefits:

• Detect multiple targets – powerful multiplexed formulation allows the detection and quantification of multiple targets in one reaction. Inclusion multiple targets for the same organism can future proof pathogen detection assays.
• Improved assay performance – excellent sensitivity and specificity can enhance existing and new RT-qPCR assays. 
• Save time - less time per run means lab efficiency and throughput can be improved.
• Improved confidence in results – robust and reliable amplification generates results you can rely on, without the fear of carryover contamination.
• Minimal optimisation required – we’ve done the work, so you don’t have to: easily amplify the most challenging of targets.

Think that MegaMix Platinum Probe RT-qPCR might improve your workflow? Request your free sample here. Microzone’s expert team are on-hand to provide any assistance you may need to implement MegaMix Platinum Probe RT-qPCR.

Working with DNA? MegaMix Platinum Probe qPCR is also available, boasting the same market leading performance for DNA amplification, click here for more information or click here to request a sample.