In a field where time-sensitive pathogen detection and sample throughput can make all the difference, molecular microbiologists face a persistent challenge: how to speed up PCR without sacrificing sensitivity and specificity. A new application note offers a solution, introducing MegaMix Emerald qPCR Mastermix—a formulation that slashes PCR run times from 1.5 hours to just over 30 minutes while maintaining high performance.

MegaMix Emerald qPCR Mastermix is designed for ultra-fast cycling, demonstrating remarkable efficiency with both purified DNA and crude bacterial lysates, even in samples from tough-to-lyse organisms like Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Paired with Microzone’s microLYSIS Plus lysis buffer, this innovative combination can help labs eliminate time-intensive DNA purification methods, delivering robust amplification with minimal environmental impact.

For molecular microbiologists aiming to maximize productivity and streamline workflows in pathogen detection, clinical diagnostics, and food safety applications, the MegaMix Emerald qPCR Mastermix may prove to be an essential tool. 

Download the full application note to explore detailed methodologies, standard curve efficiencies, and comparisons between conventional and ultra-fast qPCR profiles using MegaMix Emerald qPCR Mastermix.