Creative Diagnostics, a global leading supplier of of raw materials, antibodies, and reagents for bio-technology industry, has successfully developed a SARS-CoV-2 pseudovirus with HIV lentiviral vector. The Luciferase luminescence value reaches 106 RLU after the pseudovirus infection, which can meet the requirement for SARS-CoV-2 neutralization assay and screening of neutralization antibodies or serum.

Pseudovirus refers to a retrovirus that can integrate the envelope glycoprotein of another virus to form a virus with an exogenous viral envelope, and the genome retains the characteristics of the retrovirus itself. Some viruses, such as the new coronavirus SARS-CoV-2 (2019-nCoV), SARS-CoV, Ebola virus, and H5N1, are highly infectious and highly pathogenic, which have brought great difficulty and danger to the screening of neutralizing antibodies. Compared with natural virus, the pseudovirus can only infect cells in a single round, has broad host range, high titer, and is not easily inactivated by serum complement.

In the pseudovirus luciferase assay (PVLA), the inhibition of viral entry into cells by NtAb is correlated to the decreased levels of luciferase signals in the cells. This method is superior to the conventional assay because of its simplicity, higher sensitivity, and accuracy, suitability for high-throughput experiments.

In addition, no live virus is used during the test. Therefore, this method could be used as an alternative for safely conducting serologic studies in a rapid response in assessing the threat posed by SARS-CoV-2.

Creative Diagnostics now offers SARS-CoV-2 Pseudovirus Neutralization Assay products and related services for the research communities. “This is a powerful new addition to our coronavirus research tools portfolio. During this period, we are very closely following the changing situation on a daily basis and we support scientists to control or prevent infections with our selected antibodies, proteins, and detection kits which supported by detailed data,” said Dr. Jessica Waldorf, chief scientific officer of R&D department of Creative Diagnostics. 

Creative Diagnostics also offers various other SARS-CoV-2 related products for scientific uses, such as SARS-CoV-2 Antigens and Antibodies, Pseudotyped Luciferase rSARS-CoV-2 Spike (COV-PS01), Pseudotyped GFP rSARS-CoV-2 Spike (COV-PS02), Human ACE2 Stable Cell Line - HEK293T, and SARS-CoV-2 Inhibitor Screening ELISA Kit. 

“We’re glad to offer such support and expertise to our research community and collaborators to help them make new scientific discoveries. It is critical for us to offer such research tools for our customers during the COVID-19 pandemic, and these SARS-CoV-2 related services and products are necessary to be available to them as it's needed urgently. We’ll continue to help researchers make the most effective choices by providing more of our future products." added Jessica. 

If you want to know more details of SARS-CoV-2 Pseudovirus Neutralization Assay or any other research materials, please visit Creative Diagnostics' website or send an inquiry via the 'Request Information' option provided below.