The identification of the Enterobacteriaceae is complicated and has been traditionally based on biochemical and antigenic characteristics. With Enterotest ID, using a method well known by microbiologists worldwide, stabbing the butt and smearing a slant, six biochemical reactions are performed in a simple tube as well as providing bacterial growth for further investigative tests.

The media tube contains two layers and a paper cap for indol determination.

• Upper layer(3 ml): a butt and a slant with dextrose, ONPG, enrichments, ferrous sulphate, sodium thiosulfate, agar and phenol-red
• Lower layer (2 ml): semi-solid media, containing urea, enrichments, sodium chloride, agar and phenol-red
• Paper cap: soaked with a solution of 4-dimethyl-aminobenzaldehyde for the indol test.

6 different reactions and motility:

• Indol production
• ONPG (hydrolysis) degradation
• Glucose fermentation (acidity without gas)
• Glucose fermentation with CO2 gas formation
• Hydrogen Sulfide production
• Urea (hydrolysis) degradation
• Motility

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