This Bruker Daltonics video shows the steps required to use the MALDI Biotyper for identification of food isolates. Taking an isolated colony from either selective or non-selective agar plates, the proteomic profile is matched to the reference library and identification results are in accordance with the ISO 17025 standard, the MALDI Biotyper is validated by MicroVal according to the ISO 16140 part 6 standard and is approved official method of analysis by AOAC international, confirmation and identification results are provided directly with no need for additional data interpretation.