The Monocyte Activation Test (MAT) is an in vitro pyrogen detection assay that can detect both endotoxin and non-endotoxin pyrogens (NEPs).

Conventional MAT assays utilize PBMCs (Peripheral Blood Mononuclear Cells) and an ELISA readout, which may lead to lot-to-lot variances, can take up to two days to obtain a result, and require multiple pipetting and washing steps; however, the novel LumiMAT™ assay utilizes an introduced luciferase reporter gene to initiate a response to activated NF-κB protein to achieve a rapid, robust, and highly sensitive assay.

In the near future, the current pyrogen testing method, RPT (Rabbit Pyrogen Test), will be replaced by MAT (Monocyte Activation Test) as a more reliable, sustainable, and environmentally friendly method of pyrogen detection; however, MAT testing using PBMC (Peripheral Blood Mononuclear Cells) and ELISA (Enzyme-Linked Immunosorbent Assay) raises concerns about data variability, PBMC availability, and required assay time that are effectively mitigated with FUJIFILM Wako’s LumiMAT™ system.

The advantages of the LumiMAT™ assay are that it is ELISA-free, non-serum based, easy to handle, and provides a significantly shorter reaction time, as this system does not need to wait for IL-6 release.

Features

• Easy-to-use
• Stable performance (Less lot-to-lot variation)
• Animal free
• Shorter time to result (4-8 Hours compared to 1-2 days)
• High sensitivity, high accuracy, and low CV
• Detects a variety of pyrogens (Endotoxin and NEPs)

To learn more, visit FUJIFILM Wako or use the Request Information button below to contact the supplier.