The MAT (Monocyte Activation Test) functions as an in vitro method for detecting both endotoxin and non-endotoxin pyrogens (NEPs) in laboratory settings.

Traditional MAT procedures use PBMCs (Peripheral Blood Mononuclear Cells) combined with ELISA measurements, resulting in batch variations, extended processing times of up to 48 hours, and multiple manual handling steps; in contrast, the innovative LumiMAT™ system incorporates a luciferase reporter gene that responds to activated NF-κB protein, delivering quick, reliable, and highly precise results.

Looking ahead, the traditional RPT (Rabbit Pyrogen Test) will transition to MAT as the preferred method for pyrogen detection, offering improved reliability, sustainability, and ecological benefits. MAT testing utilizing PBMC  and ELISA presents challenges regarding result consistency, PBMC sourcing, and test duration, which FUJIFILM Wako's LumiMAT™ technology effectively addresses.

The LumiMAT™ system offers distinct benefits including ELISA independence, serum-free operation, simplified handling procedures, and reduced processing time, as it eliminates the need to wait for IL-6 release.

Features and Benefits Include:

• User-friendly operation
• Consistent results (Minimal batch variation)
• No animal testing required
• Faster results (4-8 hours versus 1-2 days)
• Superior precision, accuracy, and reproducibility
• Identifies multiple pyrogen types (Endotoxin and NEPs)

To learn more, visit FUJIFILM Wako or use the Request Information button below to contact the supplier.