Novel Coronavirus (2019 nCoV) Real-Time Multiplex RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 in Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (bronchoalveolar lavage fluid BALF, etc.) by real-time PCR systems.

The principle of this real-time detection is based on the fluorogenic 5’ nuclease assay.  During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA.  This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored in real-time by the PCR detection system.

The PCR Cycler device Mini8 Plus allows for battery-supplied diagnoses: Portable Molecular Diagnostic Solution qPCR Anytime/Anywhere!!