The principle behind the RAPID'L.mono medium relies on the specific detection of L. monocytogenes phospholipase C (PIPLC) and on the inability of this species to metabolize xylose.

After 24 hours of incubation, Listeria monocytogenes forms characteristic blue (pale blue, grey-blue to dark blue) colonies without a yellow halo. Colonies formed by other species of Listeria are white, with or without a yellow halo.

The characteristic of the species Listeria ivanovii, infrequently found in food matrices, should be noted: they present blue-green colonies with a yellow halo (indicating xylose positive ). This halo can appear after 24 to 48 hours of incubation.

The selective solution in the medium inhibits interfering flora (Gram-positive and Gram-negative bacteria, yeasts and molds).

RAPID'L.mono: 24h enrichment + 24h detection + 6h confirmation

Click here for more information on RAPID'L.mono chromogenic plating media specifically for detection and enumeration of L.monocytogenes

Now, for faster confirmation and differentiation of Listeria monocytogenes and Listeria ivanovii, the Rhamnose test was certified by AFNOR certification for a confirmation of positive results from RAPID'L.mono strains in 6 hours.

• The easiest confirmation of Listeria monocytogenes
• A simple protocol: 1 broth
  
• Fast results: 6h
  
• Flexible protocol: reading possible after 72h incubation
  
• Differentiation of Listeria monocytogenes and Listeria ivanovii
  
• AFNOR certified