Gram-color Modified and Tb-fluor: Two Phenol-free Staining Kits Safe, Clean and Easy
| Up to now, phenol was the first choice among additives to staining solutions. But because of its numerous noxious qualities, safety measures had to be taken during its application. Merck has developed a modified and phenol-free Gram's staining kit and phenol-free Tb-fluor staining kit that satisfies even the highest diagnostic demands and are simple to handle and user-friendly at the same time. |
Merck's Gram-color Modified, Phenol-free | Merck's Tb-fluor, Phenol-free |
| The kit includes a modified crystal violet solution that is free of phenol. The staining result in Gram-positive bacteria can be enhanced further still by mixing 1:1 with sodium hydrogen carbonate. The iodine solution is an iodine/potassium iodine solution stabilized with PVP that reduces the evaporation of iodine and keeps it more stable. The decolorizing solution is a mixture of ethanol and acetone, which ensures a secure differentiation of the bacteria. Counterstaining is enacted using a phenol-free fuchsin solution, which make the contrast to the intensively colored Gram-positive bacteria even more apparent. Available product: Merck Cat. No. 1.01603 Gram-color modified, phenol-free [2 x 100 ml, 3 x 200 ml] | Mycobacteria are difficult to stain because of the high proportion of lipid and wax in their cell walls. Once stained, acid fast mycobacteria keep their colouring even after treatment with strong decolourizing solutions as HCl ethanol. Our modified staining solution replaces the phenol by an active and less hazardous ingredient. The kit contains the phenol-free auramine O and rhodamine B in the staining solution for more flexibility and optical brilliance. A positive result is visible with red-orange or yellow-green fluorescence. To be able to gain an optimal impression of the fluorescence, the intrinsic fluorescence of the background is suppressed by potassium permanganate. The Tb-fluor phenol-free is suitable for paraffin sections and clinical specimen such as sputum, bronchial lavage, urine and similar. Available product: Merck Cat. No. 1.01597 Tb-fluor phenol-free kit [4 x 200 ml] |
For further information about the wide range of products available from Merck Millipore for bacteria detection by staining, please contact please contact Merck Millipore (see details above) or EMD Chemicals in the USA or click the link below.
NOTE: This item is from our 'historic' database and may contain information which is not up to date.
Source : Merck Millipore View Company Information
Posted on August 2, 2007
Get our eNewsletter
Over 7,000 microbiology professionals get our weekly eNewsletter - subscribe now and find out why!
LATEST MICROBIOLOGY NEWS
- Coming Soon: Home Testing with Liquid and Dry Swabs by MWE
- Breakthrough in qPCR Efficiency: Cut Your PCR Run Time by 75% with MegaMix Emerald qPCR Mastermix
- Fighting Resistance: Innovative Solutions Against Resistant Candida
- Preanalytics in Focus: Choosing the Right Swab, Transport, and Automation
- Consistent Quality for At-home or Point-of-care Swab Testing
- The Evolution of Swabs: From Collection Tools to Active Diagnostic Enablers
- Preanalytics: Choosing The Right Swab, Transport And Automation - a rapidmicrobiology Special Focus
- Transforming Food Safety with Real-Time Results via Mobile
- Addressing Subjective Readings and Manual Workflows in Allergen Testing
- Be Prepared this Season with Puritan® Specimen Collection Supplies
MICROBIOLOGY EVENTS
- Confience Webinar - Steps to Selecting the Right LIMS Partner
- PharmaLab Congress and Exhibition 2024
- Alternative and Rapid Microbiological Methods
- Setting Shelf life: How To Do It Better
- XXII Workshop on Rapid Methods and Automation in Food Microbiology (MRAMA) - DYCFung Memorial
- BOS Manchester 2024
- Optimizing ISO Method Testing with Rapid Solutions
- Saving Time and Money With Alicyclobacillus Rapid Microbiology Method
- 9th Annual Microbiome Movement Summit Europe
- 2025 QC Micro Summit