ANSR™ for Listeria Receives Added AOAC Matrix Approvals

Neogen Corporation has received a matrix extension for its Performance Tested MethodSM Certification (PTM #101202) from the AOAC Research Institute for its ANSR™ for Listeria assay.

In addition to the original claims, the following matrices are now included in the approval: pasteurized milk, Mexican-style cheese, ice cream, smoked salmon, lettuce, cantaloupe, guacamole, and pasteurized liquid egg. The original AOAC-RI approval from October 2012 covered the use of the ANSR system to detect Listeria in environmental matrices that included sponge or swab samples from stainless steel, plastic, ceramic tile, sealed concrete, and rubber environmental surfaces.

The issuance of this certificate from the AOAC-RI independently confirms the performance of the assay as equivalent to that of the FDA or USDA reference methods for Listeria detection in the stated matrices.

The ANSR system uses an innovative isothermal DNA amplification process to amplify DNA to detectable levels and fluorescent molecular beacon technology for detection of the pathogen target. Combined with ANSR's single enrichment step, Neogen's pathogen detection method can provide DNA-definitive results for Listeria in as little as 16 hours from the time the sample is taken.

Unlike PCR-based methods, ANSR requires only a single reaction temperature, which completely eliminates the time-consuming heating and cooling cycles of older methods. The ANSR system's small benchtop footprint and extremely simple test procedure make it an easy fit in any laboratory workflow. Incorporating an internal control for each assay, ANSR results are DNA definitive as well as rapid, providing a high level of confidence in the results.