Invitrogen's RNA UltraSense™ One-Step Quantitative RT-PCR System

For amplification of RNA viruses (Newcastle, Avian, West Nile, and others) and ultra-low abundance transcripts with SuperScript™ III RT and Platinum® Taq DNA Polymerase.

Using the RNA UltraSense™ One-Step qRT-PCR System you'll get

· The highest sensitivity
· High yield and specificity
· Success with RNA secondary structure
· Ease of Use

Ultra-sensitive real-time RT-PCR
The RNA UltraSense™ One-Step Quantitative RT-PCR System is specially designed for amplification and real-time detection of RNA viruses and ultra-low abundance transcripts

Optimized enzyme blend for superior performance
The RNA UltraSense™ One-Step Quantitative RT-PCR System combines two industry-leading enzymes: By incorporating SuperScript™ III RT and Platinum® Taq DNA Polymerase, the RNA UltraSense™ System offers the most sensitive, specific one-step system available for real-time qRT-PCR.

Ultra-concentrated system for sensitive amplification of low-abundance RNA
The RNA UltraSense™ System is particularly useful for the study of rare transcripts or for amplification of dilute templates. The 5X qRT-PCR reaction mix permits ~70% of the reaction mixture volume to be added to your sample - 2.5X more concentrated than other quantitative RT-PCR systems. As little as 25 pg/µl total RNA can be detected with a PCR efficiency of 97% (Figure 1).

Figure 1 - High-sensitivity RNA detection with high qRT-PCR efficiency Amplification of mChAT from Brain RNA

Total RNA from mouse brain was diluted to 25 ng/µl, 2.5 ng/µl, 0.25 ng/µl and 25 pg/µl. Thirteen microliters of each dilution were added to triplicate 20-µl reactions. The RNA UltraSense™ One-Step qRT-PCR System was able to amplify the rare ChAT message from RNA samples as dilute as 25 pg/µl with a PCR efficiency of 97%