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AOAC RI Approval for RIDA®COUNT E. coli/Coliform (R1007)

The RIDA®COUNT E.coli/Coliform (Art.No.R1007) test card has been successfully validated and approved by the AOAC Research Institute (license number: 070901). The performance of this test card has been reviewed by the institute's Performance Tested Methods^SM Program and was found to perform as stated by the manufacturer for meat and meat products.

The RIDA®COUNT test kit range for food pathogens are culture medium sheets presented in a ready to use test card format. The card is multi-layered, built up from a base overlaid by a nutrient layer and topped with a layer of inert polymer to absorb the test liquid. A non-woven fabric layer makes up the final top surface and ensures the structural stability of the test card. Colony counting is made easier by a plastic foil covering that has an integrated counting grid.

The principle of all the different RIDA®COUNT test cards is based on including specific chromogens into modified standard media. The nutrient composition of the RIDA®COUNT E. coli/Coliform test consists of a modified VRB (Violet Red Bile) medium. Non-coliforms are inhibited by the bile salts in the medium. A coliform specific enzyme, is converted by any coliforms present into a blue dye which leads to the formation of blue or blue-green colonies.

Any E. coli colonies present will reduce another E.coli specific chromogen to form a red-violet dye which combines with the blue dye and leads to the development of purple or indigo coloured colonies.

This system enables easy detection and differentiation of E. coli (red-violet colonies) and other coliform bacteria (blue or blue-green colonies) after an incubation time of 24 h at 35±1 °C.

NOTE: This item is from our 'historic' database and may contain information which is not up to date.

Source : R-Biopharm AG View Company Information

Posted on February 8, 2010