Thermo Scientific Limited has extended the Thermo Scientific range of products for the growth, isolation and identification of Vibrio cholerae and other Vibrio species to include Alkaline Peptone Water (CM1028). This liquid medium is recommended for the enrichment of Vibrio species from food and water samples.




Thermo Scientific Alkaline Peptone Water can assist in the detection of Vibrio cholerae and Vibrio species in food and water samples.

Twelve species of Vibrio have been incriminated in gastrointestinal and extra-intestinal diseases in man, the most important of which is Vibrio cholerae. Disease is spread through contaminated water and food. Vibrio cholerae O1 can survive on a variety of foodstuffs for up to 5 days at ambient temperature and for up to 10 days at 5-10°C (ref1). It can also survive freezing.

Food or water samples may contain low levels of the bacterium. Pre-enrichment in Alkaline Peptone Water allows Vibrio species to grow to detectable levels prior to plating on a suitable culture medium, such as TCBS (CM0333) or Blood Agar. The 2.0% (w/v) sodium chloride in Alkaline Peptone Water promotes the growth of V. cholerae, whilst its alkalinity inhibits most unwanted background flora.

Thermo Scientific Alkaline Peptone Water complies with the formulation recommended for the isolation of Vibrio species in several recognized international food and water standards, including FDA/BAM (ref2), APHA (ref3), ISO 8914:1990 (ref4) and the UK Environment Agency's Water Testing Methods (ref5).

For further information about Alkaline Peptone Water and other products for the growth and identification of Vibrio species available from Thermo Scientific please see contact details above.


References:
1.WHO Fact Sheet No 107 (March 2000), Cholera.
2.FDA/BAM, 8th edition (1995), Chapter 9: Vibrio cholerae, V.
parahaemolyticus, V. vulnificus and other Vibrio spp.
3.American Public Health Association (APHA), Chapter 28: VIBRIO; AOAC
Official Method 988.20 Detection of Vibrio cholerae in Oysters
4.ISO 8914:1990, Detection of Vibrio parahaemolyticus.
5.Environment Agency, (2002) The Microbiology of Drinking Water Part 10,
Methods for the Isolation of Yersinia, Vibrio and Campylobacter by Selective
Enrichment.