E.coli are natural inhabitants of the intestinal tract of warm-blooded animals, their presence in water samples is an indication of fecal pollution and the possible presence of other enteric pathogens. This test for E. coli can be applied to ambient waters or other waters matrices.

Since a wide range of sample volumes or dilutions can be analyzed by the MF technique, a wide range of E. coli levels in water can be detected and enumerated. The KwikCount^® MF EC medium (Roth Bioscience LLC, Goshen, Indiana) can be used to detect and enumerate fluorescent E. coli within 7 to 9 hours of incubation at 41±°C.

KwikCount® MF EC is now EPA approved for testing E.coli in ambient waters. KwikCount^® MF EC is a membrane filter (MF) media that detect E.coli on the same day as early as 7-9 hours under long-wave UV light and beginning about 14-16 hours of incubation, the E.coli colonies will not only fluoresce in UV light but will also appear as blue/purple colonies in ambient light.

This phenomenon of a coloring compound having two unique properties simultaneously has been trademarked as a Duogen®. The KwikCount^® method incorporates and includes the advantages of utilizing a Duogen^®. When the blue fluorescence has dissipated, the E.coli colonies will continue to be present as blue/purple colonies that may continue in growth and intensify color.

Visit the Roth Bioscience LLC website or use the Request Information button below to email the company directly.