ATCC has developed a vector to express green fluorescent protein (GFP) in Gram-negative bacterial strains to support basic and applied prokaryotic research, including:

• Escherichia coli (ATCC^® 25922GFP™)
• Pseudomonas aeruginosa (ATCC^® 10145GFP™ and 15692GFP™)
• Salmonella enterica subsp. enterica serovar Typhimurium (ATCC^® 14028GFP™)
• Shigella flexneri (ATCC^® 12022GFP™)

Each clone contains a multicopy vector encoding the green fluorescent protein GFPmut3, which is expressed under the control of a lacZ promoter. This construct has been designed for use in a number of analyses, including imaging studies, host-pathogen interactions, Quality Control, and fluorescence-activated cell sorting (FACS).

To learn more about the properties of each construct, or to order these strains, please visit us online at www.atcc.org/reporters.