The Liofilchem MIC Test Strip is a quantitative assay for determining the Minimum Inhibitory Concentration (MIC) of antimicrobial agents against microorganisms to indicate appropriate patient treatment and for identifying resistance patterns.

Liofilchem MIC Test Strips are made of special high quality paper impregnated with a predefined concentration gradient of antibiotic, across 15 two-fold dilutions like those of a conventional MIC method.

On one side of the strip a MIC scale in µg/mL is displayed and a code that identifies the antimicrobial agent. Liofilchem MIC Test Strips are available in a large variety of antibiotic configurations. Each configuration is available in blister packs of 30 and 100 tests.

METHOD PRINCIPLE When the Liofilchem MIC Test Strip is applied onto an inoculated agar surface, the preformed exponential gradient of antimicrobial agent is transferred into the agar matrix. After 18 hours incubation or longer, a symmetrical inhibition ellipse centered along the strip is formed. The MIC is read directly from the scale in terms of µg/mL, at the point where the edge of the inhibition ellipse intersects with the MIC Test Strip.

Bibliography

• Performance Standards for Antimicrobial Susceptibility Testing. NCCLS M100-S Series , latest annual edition.
• Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically. NCCLS M7-A6, 2003.
• Methods for Dilution Antimicrobial Susceptibility Testing of Anaerobic Bacteria. NCCLS M11-A6, 2004.

Liofilchem started manufacturing culture media in 1983. Today, Liofilchem continues to lead the Italian microbiological industry in innovation with its microbiological identification systems, used in clinical, industrial, research, and academic laboratories around the world.

Liofilchem also produces dehydrated culture media, selective supplements, ready to use culture media in petri dish, tube, bottle and dip-slide, microbial identification and susceptibility test systems, antibiotic discs in cartridges, systems for the chemical and microbial screening in food, sterilization process control systems.